human kidney tubular hk2 cell line  (ATCC)

Journal: Antioxidants (Basel, Switzerland)

Article Title: Nephroprotective Effects of Synthetic Flavonoid Hidrosmin in Experimental Diabetic Nephropathy.

doi: 10.3390/antiox10121920

Figure Lengend Snippet: Figure 1. Hidrosmin-related effects on viability and cytotoxicity of HK2 cells: (a) MTT cell viability at 24, 48, and 72 h of incubation in medium with 0.5% FBS containing hidrosmin (H, 0.3 and 1 mM), vehicle of 1mM hidrosmin (0.5% DMSO), 20% FBS (positive control, C+), and 10% DMSO (negative control, C−); (b) BrdU cell proliferation assay at 24, 48, and 72 h incubation in medium with 10% FBS containing hidrosmin (H, 0.1, 0.3 and 1 mM), vehicle (0.5% DMSO), 20% FBS (C+) and 10% DMSO (C−). Bars represent the mean ± SEM of 6 experiments. *** p < 0.001 vs. basal (black line).

Article Snippet: Human kidney 2 (HK2) proximal tubular cell line (ATCC Cat# CRL-2190, RRID:CVCL_0302; Manassas, VA, USA) was maintained in RPMI supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM L-glutamine (Sigma-Aldrich, Saint Louis, MO, USA), 1% insulin–transferrin–selenium (Thermo Fisher Scientific) and 36 ng/mL hydrocortisone (Sigma-Aldrich).

Techniques: Incubation, Positive Control, Negative Control, BrdU Cell Proliferation Assay

Journal: Antioxidants (Basel, Switzerland)

Article Title: Nephroprotective Effects of Synthetic Flavonoid Hidrosmin in Experimental Diabetic Nephropathy.

doi: 10.3390/antiox10121920

Figure Lengend Snippet: Figure 2. Effects of hidrosmin on inflammation and oxidative stress gene expression in HK2 cells. Real-time PCR analysis in HK2 cells exposed to medium with 0.5% FBS (B, basal) containing high-glucose (HG, 24 h) (a,b) or cytokines (Cyt, 6 and 24 h) (c,d) in the absence/presence of hidrosmin (H0.1, H0.3 and H1, mM doses) or vehicle of its highest dose (0.5% DMSO). Bars represent the mean ± SEM of 3–4 experiments in duplicate. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. basal (B); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. stimulus (HG or Cyt).

Article Snippet: Human kidney 2 (HK2) proximal tubular cell line (ATCC Cat# CRL-2190, RRID:CVCL_0302; Manassas, VA, USA) was maintained in RPMI supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM L-glutamine (Sigma-Aldrich, Saint Louis, MO, USA), 1% insulin–transferrin–selenium (Thermo Fisher Scientific) and 36 ng/mL hydrocortisone (Sigma-Aldrich).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: Measurement of MDA and nitric oxide after ISL treatment upon LPS induction. LPS induce AKI mice models were developed by intraperitoneal ( i.p. ) LPS injection. A total of 30 mice were randomly divided into six groups ( n = 5): control, ISL, Fer, LPS, LPS plus ISL, and LPS plus Fer. An intraperitoneal injection of LPS (10 mg/kg) was made to induce septic AKI. ISL was administered via gavage at 50 mg/kg 30 min before LPS injection. HK2 cells were treated with 50 μM or 100 μM ISL for 5 h, before septic AKI was induced using 2 μg/mL LPS. Cells were collected 24 h after LPS inducing. And the cell experiments were repeated at three times. (A) MDA measurement of mice kidney tissue homogenate. (B) MDA measurement of HK2 cell homogenate. (C) Nitric Oxide Assay of murine serum. (D) Nitric Oxide Assay of HK2 cell supernatant. ‘*’ means compared with the LPS group and p < 0.05. ‘**’ means compared with the LPS group and p < 0.01. ‘***’ means compared with the LPS group and p < 0.001. ‘#’ means compared with the control group and p < 0.05. ‘##’ means compared with the control group and p < 0.01. ‘###’ means compared with the control group and p < 0.001.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques: Injection, Control, Nitric Oxide Assay

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: ISL inhibited Fe 2+ and lipid peroxidation accumulation in LPS-stimulated cells. HK2 cells were treated with 50 μM or 100 μM ISL for 5 h, before septic AKI was induced using 2 μg/mL LPS. Cells were collected 24 h after LPS induction. FerroOrange was used as fluorescent probe to measure the level of Fe 2+ in HK2 cells. Liperfluo was employed to detect the level of lipid peroxidation in HK2 cells. Mito-Ferrogreen could further detect Fe 2+ ions in the mitochondria of HK2 cells. Magnification: ×60 oil.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques:

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: ISL inhibited the expression of HMGB1 and increased the expression of GPX4 both in vivo and in vitro following LPS stimulation. (A) Western blot assay about HMGB1 and GPX4 in murine kidney tissues. (B) Western blot assay about HMGB1 and GPX4 in HK2 cells.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques: Expressing, In Vivo, In Vitro, Western Blot

Journal: Renal Failure

Article Title: Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis

doi: 10.1080/0886022X.2021.2003208

Figure Lengend Snippet: ISL reduced the expression of NOCA4 both in vivo and in vitro upon LPS induction (A) Western blot assay about NCOA4 in murine kidney tissues. (B) Western blot assay about NCOA4 in HK2 cells. (C) Immunohistochemical staining with NCOA4 in mice kidney tissues. Magnification for immunohistochemical staining: ×400.

Article Snippet: Human kidney epithelial tubular cell line HK2 (ATCC® CRL-2190TM) was grown in DME/F-12 medium (SH30023.01, HyClone, Logan, UT), supplemented with 10% FBS (Gibco, Life Technologies, Lofer, Austria), 100 units/mL penicillin, and 100 units/mL streptomycin (1705694, HyClone, Logan, UT), at 37 °C in a 5% carbon dioxide humidified incubator.

Techniques: Expressing, In Vivo, In Vitro, Western Blot, Immunohistochemical staining, Staining

Journal: Central-European Journal of Immunology

Article Title: Resveratrol alleviates sepsis-induced acute kidney injury by deactivating the lncRNA MALAT1/MiR-205 axis

doi: 10.5114/ceji.2021.109195

Figure Lengend Snippet: Primers for genes

Article Snippet: Human kidney proximal tubular epithelial cell 2 (HK2) cell line, purchased from American Type Culture Collection (ATCC, USA), was inoculated in minimum Eagle’s medium (MEM), which contained 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids (NEAA), under an atmosphere of 37°C and 5% CO 2 .

Techniques:

Journal: Central-European Journal of Immunology

Article Title: Resveratrol alleviates sepsis-induced acute kidney injury by deactivating the lncRNA MALAT1/MiR-205 axis

doi: 10.5114/ceji.2021.109195

Figure Lengend Snippet: Resveratrol reduced proliferation and inflammatory response of HK2 cells by regulating lncRNA MALAT1 and miR-205. A ) MALAT1 expression was determined in HK2 cells of PBS group, LPS group and LPS + Res group. *** p < 0.001. B ) MALAT1 expression in HK2 cells was altered after transfection of si-MALAT1 and pcDNA3.1- MALAT1. *** p < 0.001. C ) Viability of HK2 cell line was determined after treatments of PBS, LPS, LPS + Res, LPS + si-MALAT1 and LPS + Res + pcDNA3.1-MALAT1. *** p < 0.001 in comparison to PBS group; ### p < 0.001 in comparison to LPS group; @@@ p < 0.001 in comparison to LPS + Res group. D-F ) TNF-α (D), IL-1β (E) and IL-6 (F) levels were compared among PBS group, LPS group, LPS + Res group, LPS + si-MALAT1 group and LPS + Res + pcDNA3.1-MALAT1 group. *** p < 0.001 in comparison to PBS group; ### p < 0.001 in comparison to LPS group; @@@ p < 0.001 in comparison to LPS + Res group. G ) MiR-205 expression in HK2 cells was measured after transfection of si-MALAT1 and pcDNA3.1-MALAT1. *** p < 0.001. H) MiR-205 expression in HK2 cells was compared among PBS group, LPS group, LPS + Res group, LPS + si-MALAT1 group and LPS + Res + pcDNA3.1-MALAT1 group. *** p < 0.001 in comparison to PBS group; ### p < 0.001 in comparison to LPS group; @@@ p < 0.001 in comparison to LPS + Res group

Article Snippet: Human kidney proximal tubular epithelial cell 2 (HK2) cell line, purchased from American Type Culture Collection (ATCC, USA), was inoculated in minimum Eagle’s medium (MEM), which contained 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids (NEAA), under an atmosphere of 37°C and 5% CO 2 .

Techniques: Expressing, Transfection, Comparison